Journal of Guangxi Normal University(Natural Science Edition) ›› 2014, Vol. 32 ›› Issue (3): 94-101.

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Genetic Diversity Analysis of Nile Tilapia Breeding Families

ZHANG Yong-de1, ZENG Lan1, BIN Shi-yu2, DU Xue-song1,2, YANG Hui-zan1, CHEN Zhong1, YU Yan-ling1, LIN Yong1   

  1. 1. Guangxi Academy of Fishery Sciences, Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Nanning Guangxi 530021, China;
    2. College of Life Sciences, Guangxi Normal University, Guilin Guangxi 541004, China
  • Received:2014-04-11 Online:2014-09-25 Published:2018-09-25

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Abstract: The genetic diversity and genetic relationship of 14 Nile tilapia Oreochromis niloticus families were analyzed using 14 microsatellite markers. The genetic analysis of 14 tilapia families showed that:Polymorphic bands could be amplified in 11 pairs of primers and the average number of alleles in each pair of primers was 8.36. 14 tilapia families had a higher genetic polymorphism. The average polymorphism information contents, the average heterozygosities and the average effective allele numbers were 0.499 3, 0.558 3 and 2.608 0, respectively. Pedigree analysis among the 14 Nile tilapia families showed that Family NT207 and NT213 had the smallest genetic distance of 0.204 4, and Family NT208 and NT210 had maximum genetic distance 0.760 9. The average Nei’s genetic identity and average genetic distance among the 14 Nile tilapia families were 0.649 6 and 0.440 2, respectively. UPGMA phylogenetic tree analysis showed that NT215, NT218, NT210 and NT211 clustered to one branch, while NT214, NT203, NT206, NT207, NT213, NT217, NT209 and NT216 clustered to another branch, while NT208 and NT201 had far genetic distance with other families, each forming a separate branch.

Key words: tilapia, microsatellite marker, genetic diversity, genetic relationship

CLC Number: 

  • Q959.483
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