EGCG棕榈酸酯体外抗HCV作用的研究

doi:10.16088/j.issn.1001-6600.2015.01.020

Journal of Guangxi Normal University(Natural Science Edition) ›› 2015, Vol. 33 ›› Issue (1): 122-126.doi: 10.16088/j.issn.1001-6600.2015.01.020

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Antiviral Effects of EGCG-P on Hepatitis C Virus in vitro

ZHANG Jie^1,2, LIU Qian-ying², SUN Shi², LIANG Ren-shan^1,2, SONG De-gui¹

1. College of Life Science, Guangxi Normal University, Guili Guangxi 541004, China;
2.Wuhan Institute of Biotechnology, Wuhan Hubei 430075, China

Received:2015-01-13 Online:2015-03-15 Published:2018-09-17

Abstract

Abstract: This research is to explore the antiviral effects of Epigallocatechin gallate palmitate (EGCG-P) on hepatitis c virus (HCV). To achieve this goal, the method of CCK 8 detecting EGCG-P toxic effect of Huh7.5.1 cell line, hepatitis C virus nucleic acid detection kit, and real-time PCR (RT-PCR) were used to study the role of antiviral drug effect. The study indicated that, when the EGCG-p concentration was from 0~50 mg/L, the antiviral effect was significant. When the concentration was 25 mg/L, virus expressing quantity was about 1/6 of the positive control. The result strongly support the conclusion that EGCG-P can efficiently inhibit the multiplication of HCV.

Key words: EGCG-P, HCV, Huh7.5.1, RT-PCR, cck-8

CLC Number:

R373.2

ZHANG Jie, LIU Qian-ying, SUN Shi, LIANG Ren-shan, SONG De-gui. Antiviral Effects of EGCG-P on Hepatitis C Virus in vitro[J].Journal of Guangxi Normal University(Natural Science Edition), 2015, 33(1): 122-126.

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