Nr4a3对乳腺癌细胞增殖、凋亡及侵袭的影响

doi:10.16088/j.issn.1001-6600.2023013001

Abstract

Abstract: Nr4a3 (nuclear receptor subfamily 4 group a member 3) belongs to the nuclear receptor subfamily and can regulate genes involved in proliferation, cell migration and apoptosis. Nr4a3 has been reported to be down-regulated in a variety of cancer cells. To explore the relationship between the expression of Nr4a3 in breast cancer tissues and the clinical prognosis of breast cancer patients, and its effect on cell proliferation and apoptosis, the expression of Nr4a3 in breast cancer tissues and its relationship with overall survival were analyzed using public databases. In 4T1 cell line, apoptosis and cell cycle were detected by flow cytometry, CCK-8 and colony formation were used to detect cell viability, cell scratch and invasion persisted to measure cell migration and invasion ability, the expression levels of cell cycle and apoptosis genes were detected by q-PCR. The results showed that the expression level of Nr4a3 was down-regulated in breast cancer tissues, which was positively correlated with the overall survival rate and recurrence-free survival rate of breast cancer patients. After Nr4a3 was knockdowned, cell proliferation was enhanced, cell apoptosis was reduced, and cell migration and invasion abilities were improved. This study reveals the potential role of Nr4a3 in breast cancer cell proliferation, apoptosis, migration and invasion, and may become a potential therapeutic target for breast cancer.

Key words: Nr4a3, breast cancer, apoptosis, immunotherapy

CLC Number: R737.9

ZHENG Wanhua, MO Siping, ZHOU Zuping, PU Shiming. Knockdown of Nr4a3 in Proliferative and Apoptotic Activity of Breast Cancer Cells[J].Journal of Guangxi Normal University(Natural Science Edition), 2024, 42(3): 189-197.

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Knockdown of Nr4a3 in Proliferative and Apoptotic Activity of Breast Cancer Cells

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