Journal of Guangxi Normal University(Natural Science Edition) ›› 2010, Vol. 28 ›› Issue (1): 68-71.

Previous Articles     Next Articles

Preparation of Recombinant Human Fibroblast Growth Factor 8a andits Effect on Chick Embryo Chorioallantoic Membrane Angiogenesis

ZHOU Fei-yan1,2, LU Liu-yan3, WEI Jian2, QIU Li-gong2, HUANG Ming1,2   

  1. 1. College of Life Science,Guangxi Normal University,Guilin Guangxi 541004,China;
    2. Guangzhou Baidi Biotechnology CO.LTD,Guangzhou Guangdong 511495,China;
    3. MABSKY Biotech Inc,Shenzhen Guangdong 518172,China
  • Received:2009-11-02 Online:2010-03-20 Published:2023-02-07

Abstract: To investigate the effect of rhFGF-8a to chick embryo chorioallantoic membrane angiogenesis.RhFGF-8a protein is over-expressed in E.coli through genetic engineering technology and insoluble components is about 50% of the total protein.RhFGF-8a protein is refoled andpurified and its purity can reach 99% identified by SDS-PAGE.The biologicalactivity of rhFGF-8a is determined by MTT and its effect on angiogenesis is identified by chick embryo chorioallantoic membrane(CAM) experiments.The results showthat the purified rhFGF-8a protein can stimulate the growth of NIH 3T3 cells and rhFGF-8aprotein has weak capacity on angiogenesis in CAM.

Key words: rhFGF-8a, chick embryo chorioallantoic membrane, angiogenesis

CLC Number: 

  • R979.1
[1] MARSH S K,BANSAL G S,ZAMMITC,et al.Increased expression of fibroblast growth factor 8 in human breast cancer[J].Oncogene,1999,18(4):1053-1060.
[2] VALTA M P,HENTUNEN T,QU Qiang,et al.Regulation of osteoblast differentiation:a novel function for fibroblast growth factor 8[J].Endocrinology,2006,147(5):2171-2182.
[3] PAYSON R A,WU J,LIU Y,et al.The human FGF-8 gene localizes on chromosome 10q24 and is subjected to induction by androgen in breast cancer cells[J].Oncogene,1996,13(1):47-53.
[4] MACARTHUR C A,LAWSHE A,SHANKAR D B,et al.FGF-8 Isoforms differ in NIH3t3 cell transforming potential[J].Cell Growth & Differentiation,1995,6(7):817-825.
[5] ZAMMIT C,COOPE R,GOMM J J,et al.Fibroblast growth factor 8 is expressed at higher levels in lactating human breast and in breast cancer[J].British Journal of Cancer,2002,86(7):1097-1103.
[6] MATTILA M M,TARKKONEN K M,SEPPANEN J A,et al.Androgen and fibroblast growth factor 8 (FGF8) downregulation of thrombospondin 1 (TSP1) in mouse breast cancer cells[J].Molecular and Celluar Endocrinology,2006,253(1/2):36-43.
[7] 国家药典委员会.中华人民共和国药典[M].北京:化学工业出版社,2005:106.
[8] 胡定慧,马润娣,于立坚.土贝母苷甲抑制血管生成[J].中国药理学通报,2003,19(6):713-715.
[9] 张维东,崔亚洲,姚成芳,等.蝎毒多肽提取物抗肿瘤血管生成作用的实验研究[J].中国药理学通报,2005,21(6):708.
[10] HERNáNDEZ-MARTíNEZ R,CASTRO-OBREGóN S,COVARRUBIAS L.Progressive interdigital cell death:regulation by the antagonistic interaction between fibroblast growth factor 8 and retinoic acid[J].Development,2009,136(21):3669-3678.
[11] MATTILA M M,HA¨RKO¨NEN P L.Role of fibroblast growth factor 8 in growth and progression of hormonal cancer[J].Cytokine & Growth Factor Reviews,2007,18(3):257-266.
[1] PENG Yan, CAI Le-jing, ZHOU Yuan-hua, LI Zhi, LIURen-lu, ZHONG Hui, ZHANG Guo-hai. In vitro Anti-tumor Effect of Plumbagin Derivative Pln-7-co(oAc) [J]. Journal of Guangxi Normal University(Natural Science Edition), 2013, 31(1): 67-71.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] CHEN Yong-qi, BAI Ke-zhao, KUANG hua, KONG Ling-jiang, LIU Mu-ren. Effect of Internal Layout on the Pedestrian Evacuation in the Classroom[J]. Journal of Guangxi Normal University(Natural Science Edition), 2011, 29(1): 1 -4 .
[2] XU Lun-hui, YE Fan. Acceleration Noise Model Based on Horizontal,Vertical and LateralAcceleration[J]. Journal of Guangxi Normal University(Natural Science Edition), 2011, 29(1): 5 -9 .
[3] YANG Li, KONG Ling-jiang. Capillary Force between Microparticles[J]. Journal of Guangxi Normal University(Natural Science Edition), 2012, 30(1): 1 -4 .
[4] HE Qing, LIU Jian, WEI Lianfu. Single-Photon Detectors as the Physical Limit Detections of Weak Electromagnetic Signals[J]. Journal of Guangxi Normal University(Natural Science Edition), 2022, 40(5): 1 -23 .
[5] BAI Ke-zhao, LUO Xu-dong, KONG Ling-jiang, LIU Mu-ren. Cellular Automaton Model of Date Transmission with Open Boundary Condition[J]. Journal of Guangxi Normal University(Natural Science Edition), 2010, 28(3): 1 -4 .
[6] XU Lun-hui, LIAO Ran-kun. Signal Phasing-Sequence Optimization of Intersection Based on Traffic Track[J]. Journal of Guangxi Normal University(Natural Science Edition), 2010, 28(3): 5 -9 .
[7] WANG Xiu-xin, QIN Li-mei, NONG Jing-hui, LIANG Zong-jin, ZHU Qi-jiang. Land Surface Temperature Retrieval with Mono-window Algorithm in Karst City[J]. Journal of Guangxi Normal University(Natural Science Edition), 2010, 28(3): 10 -14 .
[8] LI Yu-fang, ZHANG Jun-jian. Strong Consistency of the Regression Weighted Function Estimator for Negatively Associated Samples[J]. Journal of Guangxi Normal University(Natural Science Edition), 2010, 28(3): 15 -19 .
[9] JIA Bao-hua. A Strictly Stationary Associated Random Sequence Which Unsatisfythe Central Limit Theorem[J]. Journal of Guangxi Normal University(Natural Science Edition), 2010, 28(3): 20 -23 .
[10] CHEN Cui-ling, LI Ming, LIANG Jia-mei, LI Lüe. A Class of New Conjugate Gradient Method and Its Convergence Property Under the Wolfe Line Search[J]. Journal of Guangxi Normal University(Natural Science Edition), 2010, 28(3): 24 -28 .
Copyright © Journal of Guangxi Normal University(Natural Science Edition), All Rights Reserved.
Tel: 0773-5857325　E-mail: gxsdzkb@mailbox.gxnu.edu.cn
Powered by Beijing Magtech Co. Ltd