Journal of Guangxi Normal University(Natural Science Edition) ›› 2010, Vol. 28 ›› Issue (1): 58-62.

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Subcellular Distribution and Chemical Fractions of Manganese in Hyperaccumulator Polygonum pubescens Blume

DENG Hua1,2,3, LI Ming-shun1,3, CHEN Ying-xu2, YU Fang-ming1,3, CHEN Chun-qiang1,3, ZHOU Zhen-ming1,3   

  1. 1. Department of Environmental Engineering,Guangxi Normal University,Guilin Guangxi 541004,China;
    2. Department of Environmental Engineering,Zhejiang University,Hangzhou Zhejiang 310029,China;
    3. Guangxi Key Laboratory of Enbironmental Engineering,Protection and Assessment,Guilin Guangxi 541004,China
  • Received:2009-12-18 Online:2010-03-20 Published:2023-02-07

Abstract: The experiments were carried out to find out subcellular distribution characteristics and chemical forms of Mn in the leaves,stems and roots of Polygonum pubescens Blume,which is a newly found manganese hyperaccumulator.Differential centrifugation and sequential chemical extraction were used.More than 90% of total Mn was bound to cell wall and soluble fraction in theplant.The results from extraction experiments by using different extractants showed that water-extractable Mn and NaCl-extractable Mn predominated in stems,while water-extractable Mn,NaCl-extractable Mn and HCl-extractable Mn predominated in roots and leaves.With the Mn supply increasing,the percentage of Water-extractable Mn decreased while the percentage of HCl-extractable and NaCl-extractable Mn increased in the roots,and Water-extractable and HCl-extractable Mn increased in the leaves.Most of the Mn,which was accumulated by P.pubescens Blume,wasstored in the cell wall and the soluble fractions (including cytoplasm and karyon).For the chemical forms,Water,HCl and NaCl-extractable Mn predominated in the plant.

Key words: Manganese, Polygonum pubescens Blume, subcellulardistribution, chemical forms

CLC Number: 

  • X171.5
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