Journal of Guangxi Normal University(Natural Science Edition) ›› 2018, Vol. 36 ›› Issue (1): 121-125.doi: 10.16088/j.issn.1001-6600.2018.01.017

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Comparative Study on Effects of Fixation Nuclear Membrane Proteins by Different Fixative Agents in Immunofluorescence

WU Zhuoling1,2,3,LAI Yanhua1,YANG Liu1,2,3*   

  1. 1.College of Life Science,Guangxi Normal University,Guilin Guangxi 541006,China;
    2. Guangxi Universities Key Laboratory of Stem Cell and Biopharmaceutical,Guangxi Normal University,Guilin Guangxi 541004,China;3. Research Center for Biomedical Science,Guangxi Normal University,Guilin Guangxi 541004,China
  • Received:2017-01-22 Online:2018-01-20 Published:2018-07-17

Abstract: Immunofluorescence is a common method of detecting protein cell location. Different cell fixation methods may affect the staining effect. In this study, 3 different fixative agents were performed for immunofluorescence experiments on nuclear membrane protein lamin A/C and emerin. It revealed good immunofluorescence staining for 4% paraformaldehyde-fixed cells, relatively weaker lamin A/C staining than emerin for methanol fixation cells, and non-continuous dyeing around nuclear membrane for the acetone-fixed cells. In an overall consideration, 4% paraformaldehyde, one of the most commonly fixative agents, can be used for good staining effect. However, the suitable fixing method for targeted new protein is still necessary to explore due to the specific protein structure and function.

Key words: fixatives, immunofluorescence, nuclear membrane protein

CLC Number: 

  • R446.61
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