Journal of Guangxi Normal University(Natural Science Edition) ›› 2018, Vol. 36 ›› Issue (3): 68-75.doi: 10.16088/j.issn.1001-6600.2018.03.010

• Orginal Article • Previous Articles     Next Articles

Extraction and Anti-inflammatory Activities of Two Polyphenols from the Cake of Camellia oleifera Abel.

LI Guoqing1, 2, 3, XU Chengting1, 2, WANG Lixin1, 2, HUANG Jun1, 2, JIAO Bing1, 2, QIN Jiangke1, 2, *   

  1. 1.College of Chemistry and Pharmaceutical Sciences,Guangxi Normal University,Guilin Guangxi 541004,China;
    2. State Key Laboratory for Chemistry and Molecular Engenineering of Medicinal Resources, Guangxi Normal University,Guilin Guangxi 541004,China;
    3.Department of Food and Biotechnology, Guangxi Vocational and Technical College,Nanning Guangxi 530226, China
  • Received:2017-12-07 Online:2018-07-17 Published:2018-07-17

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Abstract: For providing the theoretical foundation and experiment evidence to further study on the polyphenols from the cake of Camellia oleifera Abel. and their pharmacological activities, two polyphenols compounds (kaempferol-3-O-[2-O-β-D-galactopyranosyl-6-O-α-L-rhamnopyranosyl] -β-D-glucopyranosie (1) and kaempferol-3-O-[2-O-β-D-xylopyranosyl-6-O-α-L-rhamnopyranosyl]-β-D-glucopyranoside (2)) from the cake of C. oleifera Abel. were obtained through degrease, ethanol extraction and prep-MPLC. MTT method was used to evaluate the cytotoxicity on RAW264.7 cell, establish cell model of LPS-induced inflammation and detect the anti-inflammatory activities of flavone glycosides compound 1, 2 and the mixture 3. The results of anti-inflammatory activity assay showed that the flavone glycosides compound 1, 2 and the mixture 3 were less toxic to RAW264.7 and both of them could significantly suppress the production of NO, iNOS, TNF-α in cells, which indicated that both of them had good anti-inflammatory effects and good prospect in drug research and development.

Key words: cake of Camellia oleifera Abel., polyphenols, extraction, anti-inflammatory activity

CLC Number: 

  • R284.1
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