Journal of Guangxi Normal University(Natural Science Edition) ›› 2018, Vol. 36 ›› Issue (3): 76-79.doi: 10.16088/j.issn.1001-6600.2018.03.011

• Orginal Article • Previous Articles     Next Articles

Electron Microscopy Study on the Apoptosis and Autophagy of the Hepatic Stellate Cells Induced by Total Alkaloids

MENG Chunmei1, LU Shiyin2, LIANG Yonghong2, MO Xiaomin1, LI Weidong1, HUANG Yuanjie1, CHENG Xiaojing1, SU Zhiheng2, ZHENG Hua1, *   

  1. 1.Life Sciceces Institute,Guangxi Medical University,Nanning Guangxi 530021,China;
    2.Pharmaceutical College,Guangxi Medical University,Nanning Guangxi 530021,China
  • Received:2017-06-16 Online:2018-07-17 Published:2018-07-17

Abstract: To study the effects of total alkaloids on apoptosis and autophagy of hepatic stellate cells in vitro.Cell inhibition rates which using different concentrations (0.20、0.25、0.30 g·L-1) of Dehydrocavidine group, Pakistan Martin group and Berberine group to the cultured HSC-T6 cells in vitro were detected.The ultrastructural changes of each group cells were observed by transmission electron microscopy. The results showed that, the cell inhibition rates of HSC-T6 cells induced by different drugs in three groups were dependent on concentrations;the HSC-T6 cells showed typical ultrastructural changes of apoptosis after 24h’s processing with different concentrations of the drugs,and there were different levels of the autophagy.Cavitin, Martin and berberine could induce apoptosis and autophagy of the HSC-T6 cells. There three components may be effective to anti liver fibrosis in total alkaloids of the Coptis chinensis Franch.

Key words: apoptosis, autophagy, hepatic stellate cell, ultrastructure

CLC Number: 

  • R962
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