Abstract: To investigate the role of methylprednisolone in renal fibrosis in mice,A renal fibrosis model was established by Unilateral Ureteral Obstruction (UUO) experiment. Twenty-five male Balb/c mice were randomly divided into sham operation group, model group, low dose group, medium dose group and high dose group. The drug was administered on the second day after the surgery, and 14 days later, renal pathological changes were detected by HE staining and Masson staining. Western blotting and IHC staining were used to detect the expression of Col-1, Col3A1, FN and α-SMA in the obstructed kidneys of each group and to detect the expression of TGF-β/Smad and NF-κB signaling pathways. The results showed that UUO can cause renal interstitial fibrosis. Methylprednisolone significantly attenuated the production of extracellular matrix Col-1, Col3A1, FN and α-SMA in UUO mice, and down-regulated expression of the protein in TGF-β/Smad and NF-κB signaling pathways. It can be seen that methylprednisolone has a significant improvement in renal fibrosis in mice, which reduces UUO-induced renal fibrosis by inhibiting TGF-β/Smad and NF-κB signaling pathways.

Key words: Methylprednisolone sodium succinate, UUO, TGF-β/Smad, NF-κB