Journal of Guangxi Normal University(Natural Science Edition) ›› 2013, Vol. 31 ›› Issue (4): 145-150.

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Study on Multiplication and Browning in Cephalotaxus mannii Hk.f.Callus

WANG Cheng-tao, ZHONG Qiu-ping, LI Yong-cheng   

  1. College of Food,Hainan University,Haikou Hainan 570228,China
  • Received:2013-07-10 Online:2013-12-20 Published:2018-11-26

Abstract: By determing the growth rate,PPO activity,POD activity and polyphenol content of Cephalo-taxus mannii Hk.f.callus,effects of anti-browning agents,hormones,medium and explants on the growth and browning of Cephalotaxus mannii Hk.f.callus were studied in this paper.The results showed that the browning rate of callus induced from the leaves was lower than that from the stems,and effective anti-browning were obtained when the callus was cultured in the medium added 0.5 g·L-1 PVP.The best proliferation medium for Cephalotaxus mannii Hk.f.callus was as follows:MS+NAA 4 mg·L-1+KT 0.1 mg·L-1+sucrose 30 g·L-1+carrageenan 6 g·L-1+PVP 0.5 g·L-1.

Key words: antibrowning, Cephalotaxus mannii Hk.f., callus, multiplication

CLC Number: 

  • Q943.1
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