Journal of Guangxi Normal University(Natural Science Edition) ›› 2022, Vol. 40 ›› Issue (2): 242-250.doi: 10.16088/j.issn.1001-6600.2020110901

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Effect of Dietary Branched-chain Amino Acid Ratio on Intestinal Flora of 28-63 Days Old Youxian Duck

SUN Yue1,2,3, DAI Qiuzhong2,4, JIANG Guitao2,4, HUANG Xuan2,4, LI Chuang2,4, DENG Ping2,4, SUN Tao1,3*   

  1. 1. College of Life Science, Guangxi Normal University, Guilin Guangxi 541006, China;
    2. Hunan Institute of Animal Science and Veterinary Medicine, Changsha Hunan 410131, China;
    3. Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin Guangxi 541006, China;
    4. Hunan Engineering Research Center of Poultry Production Safety, Changsha Hunan 410128, China
  • Received:2020-11-09 Revised:2021-04-02 Published:2022-05-31

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Abstract: A single factor experiment was adopted to investigate the effects of dietary branched-chain Amino Acid ratio flora of 28-63 days old Youxian duck. 504 Youxian ducks of 28 day-old and healthy and similar in weight were randomly allocated to 6 groups with 6 replicates each group and 14 in each replicate. Six diets with leucine, valine and isoleucine ratios of 1∶0.4∶0.3 (group MA), 1∶0.5∶0.4 (group MB), 1∶0.6∶0.5 (group MC), 1∶0.7∶0.6(group MD), 1∶0.8∶0.7(group ME) and 1∶0.9∶0.8 (group MF) were avilable. The experiment lasted for 35 days. At the end of the feeding experiment, one duck was selected from each replicate of each treatment to collect cecal contents, and the cecal microbial structure and flora diversity were detected and analyzed by 16S rDNA sequencing technology. The results show that: 1) There are 344 identical OTUs in the 6 groups, and only one unique OTU existed in group MD. 2) At the phylum level, the core flora are Bacteroidetes, Firmicutes and Proteobacteria. At the genus level, Bacteroides and Desulfovibrio are the dominant genera; 3) The results of comparion among the 6 groups show that, the abundance of Proteobacteria in group MD is significantly higher than that in group ME (P<0.05), and the abundance of Actinomycetes in group MF is significantly higher than that in group MA (P<0.05); 4)There is no significant difference in Ace index and Chao1 index among the 6 groups, however, the Shannon index in group MF is significantly higher than that in group MC (P<0.05), and Simpson index in group MC is significantly higher than that in group MF (P<0.01) and significantly higher than that in group MA (P<0.05). In summary, the core flora of the cecum of 63-day-old Youxian duck is Bacteroides, Firmicutes, and Proteobacteria. The proportion of branched-chain amino acids in diets significantly affects the Proteobacteria and Actinomycetes. When the ratio of mleucine, valine and isoleucine is 1∶0.9∶0.8, the microbial species diversity of the cecum in Youxian duck is the highest.

Key words: branched-chain amino acid, Youxian duck, intestinal flora, species abundance, species diversity

CLC Number: 

  • S834
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