Journal of Guangxi Normal University(Natural Science Edition) ›› 2010, Vol. 28 ›› Issue (3): 51-55.

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Interaction of Fe3O4 Magnetic Nanoparticles with Hyaluronidase

FAN Su-ping1,2, TIAN Jian-niao1,2, XIAO Ling-zhi1,2, ZHAO Yan-chun1,2   

  1. 1. Key Laboratory for the Chemistry and Molecular Engineeringof Medicinal Resources of Ministry of Education of China,Guangxi Normal University,Guilin Guangxi 541004,China;
    2. College ofChemistry and Chemical Engineering, Guangxi Normal University,Guilin Guangxi 541004,China
  • Received:2010-06-10 Online:2010-09-20 Published:2023-02-06

Abstract: The interaction of Fe3O4 magnetic nanoparticles with Hyaluronidase wasinvestigated by fluorescence,ultraviolet and circular dichroism spectral method(CD).The experimental results demonstrated that the quenching mechanism of Fe3O4magnetic nanoparticles to Hyaluronidase fluorescence was a static quenching dueto existence of non-radiative energy-transfer.The binding constant of Fe3O4 magnetic nanoparticles with Hyaluronidase is four factors of ten,which indicate there is strong association between them.The changes of the thermodynamic parameters showed that the main interaction forces are electrostatic reaction and the hydrophobic interaction between Fe3O4 magnetic nanoparticles and Hyaluronidase.At the same time,through the ultraviolet absorption and circular dichroism(CD) spectroscopy,it showed that the secondary structureof Hyaluronidase is changed induced by the binding of Fe3O4 magnetic nanoparticles.

Key words: Fe3O4 magnetic nanoparticles, hyaluronidase, fluorescence spectroscopy, ultraviolet, circular dichroism(CD)

CLC Number: 

  • O641
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