广西师范大学学报（自然科学版） ›› 2020, Vol. 38 ›› Issue (5): 71-77.doi: 10.16088/j.issn.1001-6600.2020.05.009

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广豆根内生真菌Xylaria sp. GDGJ-368代谢产物研究

覃玉月1,2, 刘晓波1,2, 徐照隆1,2, 莫土香1,2, 李俊1,2, 杨瑞云1,2*   

  1. 1.广西师范大学化学与药学学院, 广西桂林541004;
    2.广西师范大学 省部共建药用资源化学与药物分子工程国家重点实验室, 广西桂林541004
  • 收稿日期:2019-09-19 出版日期:2020-09-25 发布日期:2020-10-09
  • 通讯作者: 杨瑞云(1978—), 女, 广西桂林人, 广西师范大学研究员, 博士。E-mail: yang_rui_yun@163.com
  • 基金资助:
    国家自然科学基金(21762007); 广西自然科学基金(2018GXNSFAA281169); 药用资源化学与药物分子工程国家重点实验室资助项目(CMEMR2018-C29)

Secondary Metabolites of Endophytic Fungus Xylaria sp. GDGJ-368 from Sophora tonkinensis

QIN Yuyue1,2, LIU Xiaobo1,2, XU Zhaolong1,2, MO Tuxiang1,2, LI Jun1,2, YANG Ruiyun1,2*   

  1. 1. College of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin Guangxi 541004, China;
    2. State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, Guangxi Normal University, Guilin Guangxi 541004, China
  • Received:2019-09-19 Online:2020-09-25 Published:2020-10-09

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摘要： 对真菌GDGJ-368代谢产物的化学成分进行研究,为进一步明确广豆根内生真菌中代谢产物的特点提供实验依据,通过柱层析分离技术,从广豆根植物内生真菌GDGJ-368代谢产物中分离得到7个化合物,通过核磁波谱技术确定化合物的结构。实验结果表明:7个化合物分别为5-carboxylmellein (1)、diaporthein B (2)、piliformic (3)、cytochalasin C (4)、cytochalasin D (5)、(22E)-ergosta-6, 22-diene-3β, 5β, 8α-triol (6)、(22E,24R)-5α,8α-过氧化麦角甾-6,22-二烯-3β-醇 (7)。其中化合物2为首次从炭角菌属中分离得到,化合物4和5为首次从槐属植物内生真菌中分离得到。

关键词: 广豆根, 内生真菌, Xylaria sp., GDGJ-368, 代谢产物

Abstract: In order to provide experimental basis for further research on endophytic fungi from Sophora tonkinensis, the chemical compounds of the metabolites of GDGJ-368 had been studied. Seven compounds had been isolated from the metabolites through column chromatography, and their structures were elucidated by NMR spectroscopy. The results showed that the structures of the compounds were 5-carboxylmellein (1), diaporthein B (2), piliformic (3), cytochalasin C (4), cytochalasin D (5), (22E)-ergosta-6, 22-diene-3β, 5β, 8α-triol (6) and (22E, 24R) -5α, 8α-epidioxy-24-methyl-cholesta-6, 22-diene-3β-ol (7). Compound 2 was isolated from Xylaria sp. for the first time, and compounds 4 and 5 were isolated from the fungi which were isolated from the plants of the genus Sophora firstly.

Key words: Sophora tonkinensis, endophytic fungi, Xylaria sp., GDGJ-368, metabolite

中图分类号: 

  • O629.9
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