广西师范大学学报（自然科学版） ›› 2013, Vol. 31 ›› Issue (4): 98-102.

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一个简便灵敏测定空气中臭氧的罗丹明6G荧光法

梁爱惠, 杨铎, 林陈银, 温桂清   

  1. 广西师范大学环境与资源学院,广西桂林541004
  • 收稿日期:2013-06-15 出版日期:2013-12-20 发布日期:2018-11-26
  • 通讯作者: 梁爱惠(1965—),女,广西岑溪人,广西师范大学研究员。E-mail:ahliang2008@163.com
  • 基金资助:
    国家自然科学基金资助项目(21165005,21267004,21307017);广西自然科学基金资助项目(2013GXNSFF-A019003)

A Simple and Sensitive Fluorescence Method for Determination of Trace O3 in Air Using Rhodamine 6G

LIANG Ai-hui, YANG Duo, LIN Chen-yin, WEN Gui-qing   

  1. College of Environment and Resource,Guangxi Normal University,Guilin Guangxi 541004,China
  • Received:2013-06-15 Online:2013-12-20 Published:2018-11-26

摘要： 臭氧与KI-硼酸吸收液反应生成碘(I2),当I-过量时可与I2生成I-3。在pH4.0 HAc-NaAc缓冲液中,吸收液中的I-3与罗丹明6G(RhG)形成RhG-I3离子缔合物微粒,将荧光分子RhG包裹在微粒中使得体系在556 nm处的荧光峰强度减少,其强度减少值ΔF556 nm与O3浓度在0.97~15.4 μmol/L呈良好的线性关系,检出限为0.5 μmol/L。该法用于空气中臭氧测定,分析结果与国标方法结果一致。

关键词: 臭氧, 罗丹明6G, 缔合物微粒, 荧光法

Abstract: Ozone (O3) reacted with KI-H3BO3 absorption liquid to produce iodine (I2),when the I- excessive,it form I-3.In the pH4.0 HAc-NaAc (1.67 mmol/L) buffer solution,I-3 and RhG form RhG-I3 ion association particles that resulted in the resonance of the fluorescence peak decreases at 556 nm.The decreased value ΔF556 nm is linear to the I2 concentration in the range of 0.97~15.4 μmol/L.And the concentration of I2 is equivalent to O3,with a regression equation of ΔF556 nm=274.94C+52.03,coefficient of 0.991 8 and a detection limit of 0.5 μmol/L.This method was applied to determination of ozone in air sample,and its results were agreement with that of spectrophotometry.

Key words: ozone, rhodamin 6G, associated particle, fluorescence method

中图分类号: 

  • O657
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