Journal of Guangxi Normal University(Natural Science Edition) ›› 2024, Vol. 42 ›› Issue (3): 17-26.doi: 10.16088/j.issn.1001-6600.2023081702

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Komagataella phaffii Serves as a Model Organism for Emerging Basic Research

AI Congcong1,2, GONG Guoli1,2*, JIAO Xiaoyu1,2, TIAN Lu1,2, GAI Zhongchao1,2, GOU Jingxuan1,2, LI Hui1,2   

  1. 1. School of Biological and Pharmaceutical Sciences, Shaanxi University of Science and Technology, Xi'an Shaanxi 710021, China;
    2. School of Food Science and Engineering, Shaanxi University of Science and Technology, Xi'an Shaanxi 710021, China
  • Received:2023-08-17 Revised:2023-09-17 Published:2024-05-31

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Abstract: Komagataella phaffii has been widely used in the pharmaceutical and biotechnology industries. In recent years, its potential as a research model organism has gained attention. Although baker's yeast is the most commonly used yeast model in research, it limits our understanding of the same organism. K. phaffii, which diverged from baker's yeast 250 million years ago, evolves at a slower rate and retains characteristics of ancient yeast ancestors, making it more similar to higher eukaryotic cells. K. phaffii can efficiently assimilate methanol as the sole carbon source, making it a valuable model organism for studying molecular cell biology of eukaryotes. This article reviews the research progress of using K. phaffii as a model organism, including methanol assimilation, peroxisome formation, mating and sporulation behavior, as well as protein secretion, lipid synthesis, and cell wall formation processes. By comparing the data of K. phaffii with other yeast species such as baker's yeast, this article highlights the great potential of K. phaffii in basic research, aiming to present a comprehensive and systematic review of the research progress on K. phaffii as a model organism.

Key words: Komagataella phaffii, methyltrophic yeast, eukaryote, recombinat expression, model organism

CLC Number:  Q78
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